Non-aversive handling techniques, such as the tunnel and cup technique, reduce stress and anxiety in mice. More information on these handling methods can be found here. If using handling tunnels, mice can also be trained to voluntarily enter the tunnel (see video below). A detailed handling protocol on the voluntary tunnel handling will be provided on the Refinement Wiki soon.
Expert in non-aversive mouse handling techniques: Jane Hurst, University of Liverpool, Liverpool, United Kingdom