Anaesthesia in neonates
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We have a lot of groups looking at neurodevelopment and so far we've been able to use Isofluorane for all of the studies, but one group are planning on doing Ca2+ imaging and the Isoflurane is thought to affect the imaging so they would like to look at using an injectable protocol. They have proposed Medetomidine, Midazolam (+/- fentanyl) or Urethane, but I have no experience using these agents in neonates and wondered if anyone has experience they could share. In this case it is neonatal rats aged P6-11. The imaging would be terminal and last between 10-30 minutes and is non-invasive. There is a surgical set up which we may try and carry out under the same anaesthetic if possible.