Rotarod Test - Revision history

Werenskjold at 22:16, 31 January 2021

2021-01-31T22:16:15Z

KH191219 at 20:39, 16 March 2020

2020-03-16T20:39:14Z

KH191219 at 20:32, 16 March 2020

2020-03-16T20:32:08Z

KH191219 at 20:31, 16 March 2020

2020-03-16T20:31:36Z

KH191219 at 20:24, 16 March 2020

2020-03-16T20:24:14Z

Adriansmith at 15:22, 9 March 2020

2020-03-09T15:22:33Z

Adriansmith: Created page with "In the Rotarod Test motor coordination and balance can be investigated. For the test the mouse is placed on a rotating drum of the test apparatus on which it must walk foward..."

2020-03-09T15:22:05Z

Created page with "In the Rotarod Test motor coordination and balance can be investigated. For the test the mouse is placed on a rotating drum of the test apparatus on which it must walk foward..."

New page

In the Rotarod Test motor coordination and balance can be investigated. For the test the mouse is placed on a rotating drum of the test apparatus on which it must walk foward to stay upright.

'''Technique to transfer mice to drum'''

It is a common code of practice to place the mouse on the drum by holding it by its tail ([https://www.jove.com/video/2609/measuring-motor-coordination-in-mice Deacon 2013]), though tail handling can cause stress and anxiety in mice ([https://www.nature.com/articles/nmeth.1500 Hurst and West 2010]). However, if mice are used to be handled by a combination of [https://www.nc3rs.org.uk/how-to-pick-up-a-mouse tunnel and cup methods], they can be gently transfered from the experimenter's hand to the drum: When the mouse sits on the cupped hand, pronate the hand in an angle of 90° so that the mouse climbs on the thumb and index finger. Then, locate the hand in front of the rotating drum so that the mouse can climb on it. At the end of the test, those mice that have not fallen off the rotarod can be transferred by the cup technique to their home cage: Pronate the hand in an angle of 90° and place it in front of the mouse, allowing the animal to climb on the hand.<ref>Please note that this refinement strategy has not been fully investigated yet.</ref>

'''''Please note''' that the impact of the handling techniques on the results of the Rotarod Test has not been investigated yet.[[User:KatharinaH|KatharinaH]] ([[User talk:KatharinaH|talk]])''

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← Older revision		Revision as of 22:16, 31 January 2021
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−	In the Rotarod Test motor coordination and balance can be investigated. For the test the mouse is placed on a rotating drum of the test apparatus on which it must walk foward to stay upright.	+	In the Rotarod Test motor coordination and balance can be investigated. For the test the mouse is placed on a rotating drum of the test apparatus on which it must walk foward to stay upright.

← Older revision		Revision as of 20:39, 16 March 2020
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−	[[User:KH191219\|KH191219]] ([[User talk:KH191219\|talk]])	+	This page was created and edited by [[User:KH191219\|KH191219]] ([[User talk:KH191219\|talk]]).

← Older revision		Revision as of 20:32, 16 March 2020
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		+	[[User:KH191219\|KH191219]] ([[User talk:KH191219\|talk]])

← Older revision		Revision as of 20:31, 16 March 2020
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	'''Technique to transfer mice to drum'''		'''Technique to transfer mice to drum'''

−	It is a common code of practice to place the mouse on the drum by holding it by its tail ([https://www.jove.com/video/2609/measuring-motor-coordination-in-mice Deacon 2013]), though tail handling can cause stress and anxiety in mice ([https://www.nature.com/articles/nmeth.1500 Hurst and West 2010]). However, if mice are used to be handled by a combination of [https://www.nc3rs.org.uk/how-to-pick-up-a-mouse tunnel and cup methods], they can be gently transfered from the experimenter's hand to the drum: When the mouse sits on the cupped hand, pronate the hand in an angle of 90° so that the mouse climbs on the thumb and index finger. Then, locate the hand in front of the rotating drum so that the mouse can climb on it. At the end of the test, those mice that have not fallen off the rotarod can be transferred by the cup technique to their home cage: Pronate the hand in an angle of 90° and place it in front of the mouse, allowing the animal to climb on the hand.	+	It is a common code of practice to place the mouse on the drum by holding it by its tail ([https://www.jove.com/video/2609/measuring-motor-coordination-in-mice Deacon 2013]), though tail handling can cause stress and anxiety in mice ([https://www.nature.com/articles/nmeth.1500 Hurst and West 2010]). However, if mice are used to be handled by a combination of [https://www.nc3rs.org.uk/how-to-pick-up-a-mouse tunnel and cup methods], they can be gently transfered from the experimenter's hand to the drum: When the mouse sits on the cupped hand, pronate the hand in an angle of 90° so that the mouse climbs on the thumb and index finger. Then, locate the hand in front of the rotating drum so that the mouse can climb on it. At the end of the test, those mice that have not fallen off the rotarod can be transferred by the cup technique to their home cage: Pronate the hand in an angle of 90° and place it in front of the mouse or behind it, allowing the animal to climb on the hand.

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 '''Technique to transfer mice to drum'''
-It is a common code of practice to place the mouse on the drum by holding it by its tail ([https://www.jove.com/video/2609/measuring-motor-coordination-in-mice Deacon 2013]), though tail handling can cause stress and anxiety in mice ([https://www.nature.com/articles/nmeth.1500 Hurst and West 2010]). However, if mice are used to be handled by a combination of [https://www.nc3rs.org.uk/how-to-pick-up-a-mouse tunnel and cup methods], they can be gently transfered from the experimenter's hand to the drum: When the mouse sits on the cupped hand, pronate the hand in an angle of 90° so that the mouse climbs on the thumb and index finger. Then, locate the hand in front of the rotating drum so that the mouse can climb on it. At the end of the test, those mice that have not fallen off the rotarod can be transferred by the cup technique to their home cage: Pronate the hand in an angle of 90° and place it in front of the mouse, allowing the animal to climb on the hand.<ref>Please note that this refinement strategy has not been fully investigated yet.</ref>
+It is a common code of practice to place the mouse on the drum by holding it by its tail ([https://www.jove.com/video/2609/measuring-motor-coordination-in-mice Deacon 2013]), though tail handling can cause stress and anxiety in mice ([https://www.nature.com/articles/nmeth.1500 Hurst and West 2010]). However, if mice are used to be handled by a combination of [https://www.nc3rs.org.uk/how-to-pick-up-a-mouse tunnel and cup methods], they can be gently transfered from the experimenter's hand to the drum: When the mouse sits on the cupped hand, pronate the hand in an angle of 90° so that the mouse climbs on the thumb and index finger. Then, locate the hand in front of the rotating drum so that the mouse can climb on it. At the end of the test, those mice that have not fallen off the rotarod can be transferred by the cup technique to their home cage: Pronate the hand in an angle of 90° and place it in front of the mouse, allowing the animal to climb on the hand.
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-'''''Please note''' that the impact of the handling techniques on the results of the Rotarod Test has not been investigated yet.[[User:KatharinaH|KatharinaH]] ([[User talk:KatharinaH|talk]])''
+'''''Please note''' that the impact of the handling techniques on the results of the Rotarod Test has not been investigated yet.''
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 <references />