MDA (micropipette-guided drug administration) Method - Revision history

Adriansmith at 14:36, 22 January 2025

2025-01-22T14:36:45Z

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2025-01-22T14:36:10Z

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Adriansmith at 22:38, 1 May 2024

2024-05-01T22:38:18Z

Adriansmith at 22:36, 1 May 2024

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Adriansmith at 22:32, 1 May 2024

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2024-05-01T22:00:52Z

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PJ191219: Created page with "Pharmacological administration method for per os treatments in mice. This method can potentially be used for different drugs, the preparation of the drug solution depends on t..."

2024-04-17T12:49:23Z

Created page with "Pharmacological administration method for per os treatments in mice. This method can potentially be used for different drugs, the preparation of the drug solution depends on t..."

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Pharmacological administration method for per os treatments in mice. This method can potentially be used for different drugs, the preparation of the drug solution depends on the drug`s properties.

'''Equipment, materials:'''

Sweet condensed milk (MIGROS Kondensmilch™, Migros, Zurich, Switzerland)

Regular Tap Water

15 or 50ml Falcon Tubes

Ultrasonic water bath*

Magnetic Stirrer*

Channel p200 Micropipette

p200 Pipette Tips

*depending on characteristics of drug to be administered

'''Procedure Preparation Drug Solution**:'''

Dilute condensed milk with regular tap water in a 4:10 (condensed milk to water) ratio. This solution is used as vehicle.

Dilute the drug to be administered per os in water and sonicate for 20 min.

Mix suspended drug with vehicle solution to reach the desired concentration.

Keep drug and vehicle solution in constant agitation using a magnetic stirrer.

Drug and vehicle solution are administered at a volume of 2 ml/kg.

** this section depends on the characteristics of the drug to be administered and might need to be adapted.

'''MDA Procedure:'''

Prepare the desired amount (2 ml/kg) of drug or vehicle solution in the tip of the p200 micropipette. For training you may use vehicle solution only.

Training Day 1: Restrain mouse gently and expose it to the milk solution for the first time, by offering the pipette tip to the mouth until the mouse begins to drink.

Training Day 2: Restrain mouse solely by the tail on the metal grid of the food hopper and position the pipette continually next to the mouse’s mouth until it drinks. Animal should ingest the complete volume.

(Optional) Training Day 3-7: If necessary, repeat the procedure from training day 2 to familiarize the mouse with the milk solution even further. Animal should ingest the complete volume.

MDA (micropipette-guided drug administration): Place mouse on the metal grid of the food hopper with no or minimal restraint and offer pipette tip until the mouse voluntarily begins to drink the solution.

'''Videos of the training procedure: https://www.sciencedirect.com/science/article/pii/S0889159120302543?via%3Dihub'''

@@ Line 39: / Line 39: @@
 '''[https://www.youtube.com/watch?v=b8adBybZgzs The Rodent MDA Introductory Video] (3:34)'''
-'''See also the video clips in the reference below'''
+See also the video clips in the reference below
 '''Reference:''' Scarborough J, Mueller F, Arban R, Dorner-Ciossek C, Weber-Stadlbauer U, Rosenbrock H, Meyer U, Richetto J (2020). [https://www.sciencedirect.com/science/article/pii/S0889159120302543?via%3Dihub Preclinical validation of the micropipette-guided drug administration (MDA) method in the maternal immune activation model of neurodevelopmental disorders]. ''Brain Behav Immun''. Apr 9:S0889-1591(20)30254-3. doi: 10.1016/j.bbi.2020.04.015.

← Older revision		Revision as of 14:36, 22 January 2025
Line 35:		Line 35:


−	'''Videos of the training procedure: ~~https://www.sciencedirect.com/science/article/pii/S0889159120302543?via%3Dihub~~'''	+	'''Videos of the training procedure:'''

−	'''Reference:''' Scarborough J, Mueller F, Arban R, Dorner-Ciossek C, Weber-Stadlbauer U, Rosenbrock H, Meyer U, Richetto J (2020). Preclinical validation of the micropipette-guided drug administration (MDA) method in the maternal immune activation model of neurodevelopmental disorders. ''Brain Behav Immun''. Apr 9:S0889-1591(20)30254-3. doi: 10.1016/j.bbi.2020.04.015.	+	'''[https://www.youtube.com/watch?v=b8adBybZgzs The Rodent MDA Introductory Video] (3:34)'''
		+
		+	'''See also the video clips in the reference below'''
		+
		+	'''Reference:''' Scarborough J, Mueller F, Arban R, Dorner-Ciossek C, Weber-Stadlbauer U, Rosenbrock H, Meyer U, Richetto J (2020). [https://www.sciencedirect.com/science/article/pii/S0889159120302543?via%3Dihub Preclinical validation of the micropipette-guided drug administration (MDA) method in the maternal immune activation model of neurodevelopmental disorders]. ''Brain Behav Immun''. Apr 9:S0889-1591(20)30254-3. doi: 10.1016/j.bbi.2020.04.015.

← Older revision		Revision as of 22:38, 1 May 2024
Line 38:		Line 38:

	'''Reference:''' Scarborough J, Mueller F, Arban R, Dorner-Ciossek C, Weber-Stadlbauer U, Rosenbrock H, Meyer U, Richetto J (2020). Preclinical validation of the micropipette-guided drug administration (MDA) method in the maternal immune activation model of neurodevelopmental disorders. ''Brain Behav Immun''. Apr 9:S0889-1591(20)30254-3. doi: 10.1016/j.bbi.2020.04.015.		'''Reference:''' Scarborough J, Mueller F, Arban R, Dorner-Ciossek C, Weber-Stadlbauer U, Rosenbrock H, Meyer U, Richetto J (2020). Preclinical validation of the micropipette-guided drug administration (MDA) method in the maternal immune activation model of neurodevelopmental disorders. ''Brain Behav Immun''. Apr 9:S0889-1591(20)30254-3. doi: 10.1016/j.bbi.2020.04.015.
−
−	~~A Standard Operating Procedure for this method can be downloaded here.~~

← Older revision		Revision as of 22:36, 1 May 2024
Line 38:		Line 38:

	'''Reference:''' Scarborough J, Mueller F, Arban R, Dorner-Ciossek C, Weber-Stadlbauer U, Rosenbrock H, Meyer U, Richetto J (2020). Preclinical validation of the micropipette-guided drug administration (MDA) method in the maternal immune activation model of neurodevelopmental disorders. ''Brain Behav Immun''. Apr 9:S0889-1591(20)30254-3. doi: 10.1016/j.bbi.2020.04.015.		'''Reference:''' Scarborough J, Mueller F, Arban R, Dorner-Ciossek C, Weber-Stadlbauer U, Rosenbrock H, Meyer U, Richetto J (2020). Preclinical validation of the micropipette-guided drug administration (MDA) method in the maternal immune activation model of neurodevelopmental disorders. ''Brain Behav Immun''. Apr 9:S0889-1591(20)30254-3. doi: 10.1016/j.bbi.2020.04.015.
		+
		+	A Standard Operating Procedure for this method can be downloaded here.

← Older revision		Revision as of 22:32, 1 May 2024
Line 38:		Line 38:

	'''Reference:''' Scarborough J, Mueller F, Arban R, Dorner-Ciossek C, Weber-Stadlbauer U, Rosenbrock H, Meyer U, Richetto J (2020). Preclinical validation of the micropipette-guided drug administration (MDA) method in the maternal immune activation model of neurodevelopmental disorders. ''Brain Behav Immun''. Apr 9:S0889-1591(20)30254-3. doi: 10.1016/j.bbi.2020.04.015.		'''Reference:''' Scarborough J, Mueller F, Arban R, Dorner-Ciossek C, Weber-Stadlbauer U, Rosenbrock H, Meyer U, Richetto J (2020). Preclinical validation of the micropipette-guided drug administration (MDA) method in the maternal immune activation model of neurodevelopmental disorders. ''Brain Behav Immun''. Apr 9:S0889-1591(20)30254-3. doi: 10.1016/j.bbi.2020.04.015.
−
−	~~A Standard Operating Procedure for this method can be downloaded here.~~

← Older revision		Revision as of 22:00, 1 May 2024
Line 38:		Line 38:

	'''Reference:''' Scarborough J, Mueller F, Arban R, Dorner-Ciossek C, Weber-Stadlbauer U, Rosenbrock H, Meyer U, Richetto J (2020). Preclinical validation of the micropipette-guided drug administration (MDA) method in the maternal immune activation model of neurodevelopmental disorders. ''Brain Behav Immun''. Apr 9:S0889-1591(20)30254-3. doi: 10.1016/j.bbi.2020.04.015.		'''Reference:''' Scarborough J, Mueller F, Arban R, Dorner-Ciossek C, Weber-Stadlbauer U, Rosenbrock H, Meyer U, Richetto J (2020). Preclinical validation of the micropipette-guided drug administration (MDA) method in the maternal immune activation model of neurodevelopmental disorders. ''Brain Behav Immun''. Apr 9:S0889-1591(20)30254-3. doi: 10.1016/j.bbi.2020.04.015.
		+
		+	A Standard Operating Procedure for this method can be downloaded here.

@@ Line 1: / Line 1: @@
 Pharmacological administration method for voluntary per os treatments in mice. This method can potentially be used for different drugs, the preparation of the drug solution depends on the drug`s properties.
@@ Line 4: / Line 5: @@
 '''Equipment, materials:'''
-* Sweet condensed milk (MIGROS Kondensmilch™, Migros, Zurich, Switzerland)
+*Sweet condensed milk (MIGROS Kondensmilch™, Migros, Zurich, Switzerland)
-* Regular Tap Water
+*Regular Tap Water
-* 15 or 50ml Falcon Tubes
+*15 or 50ml Falcon Tubes
-* Ultrasonic water bath*
+*Ultrasonic water bath*
-* Magnetic Stirrer*
+*Magnetic Stirrer*
-* Channel p200 Micropipette
+*Channel p200 Micropipette
-* p200 Pipette Tips
+*p200 Pipette Tips
 <nowiki>*</nowiki> depending on characteristics of drug to be administered
@@ Line 16: / Line 17: @@
 '''Procedure Preparation Drug Solution**:'''
-* Dilute condensed milk with regular tap water in a 4:10 (condensed milk to water) ratio. This solution is used as vehicle.
+*Dilute condensed milk with regular tap water in a 4:10 (condensed milk to water) ratio. This solution is used as vehicle.
-* Dilute the drug to be administered per os in water and sonicate for 20 min.
+*Dilute the drug to be administered per os in water and sonicate for 20 min.
-* Mix suspended drug with vehicle solution to reach the desired concentration.
+*Mix suspended drug with vehicle solution to reach the desired concentration.
-* Keep drug and vehicle solution in constant agitation using a magnetic stirrer.
+*Keep drug and vehicle solution in constant agitation using a magnetic stirrer.
-* Drug and vehicle solution are administered at a volume of 2 ml/kg.
+*Drug and vehicle solution are administered at a volume of 2 ml/kg.
 <nowiki>**</nowiki> this section depends on the characteristics of the drug to be administered and might need to be adapted.
@@ Line 27: / Line 28: @@
 '''MDA Procedure:'''
-* Prepare the desired amount (2 ml/kg) of drug or vehicle solution in the tip of the p200 micropipette. For training you may use vehicle solution only.
+*Prepare the desired amount (2 ml/kg) of drug or vehicle solution in the tip of the p200 micropipette. For training you may use vehicle solution only.
-* Training Day 1: Restrain mouse gently and expose it to the milk solution for the first time, by offering the pipette tip to the mouth until the mouse begins to drink.
+*Training Day 1: Restrain mouse gently and expose it to the milk solution for the first time, by offering the pipette tip to the mouth until the mouse begins to drink.
-* Training Day 2: Restrain mouse solely by the tail on the metal grid of the food hopper and position the pipette continually next to the mouse’s mouth until it drinks. Animal should ingest the complete volume.
+*Training Day 2: Restrain mouse solely by the tail on the metal grid of the food hopper and position the pipette continually next to the mouse’s mouth until it drinks. Animal should ingest the complete volume.
-* (Optional) Training Day 3-7: If necessary, repeat the procedure from training day 2 to familiarize the mouse with the milk solution even further. Animal should ingest the complete volume.
+*(Optional) Training Day 3-7: If necessary, repeat the procedure from training day 2 to familiarize the mouse with the milk solution even further. Animal should ingest the complete volume.
-* MDA (micropipette-guided drug administration): Place mouse on the metal grid of the food hopper with no or minimal restraint and offer pipette tip until the mouse voluntarily begins to drink the solution.
+*MDA (micropipette-guided drug administration): Place mouse on the metal grid of the food hopper with no or minimal restraint and offer pipette tip until the mouse voluntarily begins to drink the solution.